Phalaenopsis orchids stand as pivotal flora within the worldwide horticultural trade, predominantly cultivated via micropropagation to ensure consistent blooming and development. The proliferation of these in vitro plants is influenced by a variety of environmental conditions, including illumination, thermal management, and moisture levels. Notably, the spectral properties of light and the flow of air within the growth container significantly impact the morphological formation and overall expansion of the in vitro cultured plants. This study conducted to investigate the best light quality for rooting of Phalaenopsis line 834 were cultured under 5 different light qualities: White (W), Red (R), Blue (B), Red+Blue (RB), and Red+Green+Blue (RGB). Phalaenopsis cv. Univivace was cultured in a temporary immersion system (TIS) to establish large-scale plant production. At this time, semi-solid and liquid culture were performed as control. The light intensity was set to 40 µmol･m-2･s-1 PPFD in all experiments. After 8 weeks of culture, fresh weight of phalaenopsis was 1447.6 mg/plant in RB, which was 1.2-fold higher than W. The number of leaves was highest in B at 5.5 and the number of roots was 3.9 in RB, 1.3-fold higher than B. The leaf area was the widest at 274.97 mm2 in RGB and leaf length and width were the highest in RGB. By culture system, the growth of shoot in TIS was better than the control, which is considered to be due to smooth ventilation in the culture vessel. However, rooting was low in TIS. It should be improved through continuous experiments. Overall, phalaenopsis grown in R, RB, and RGB, which are the high ratio of R, had better growth and rooting conditions. In the micropropagation of phalaenopsis, the treatment of light quality with increased red light and the application of the TIS are thought to contribute to the production of healthy plants during mass production.